How to Reconstitute Peptides for Nasal Spray — Spirare Supply

Video Tutorial

Reconstituting peptides for intranasal research requires a few specific steps to get the concentration right and keep your solution sterile. This guide walks through the full process, from opening your peptide vial to priming your nasal spray bottle, using the supplies included in your Spirare kit.

Everything in one box
The Spirare Supply kit includes the nasal atomizer, sterile saline, luer lock syringes, alcohol prep pads, and labels, so you're not sourcing five things from five places.
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What You Need
Your Spirare Supply kit Your peptide vial (10mg)
Step 01

Reconstitution

  1. 1. Open the saline ampoule and dump the full 5mL into the nasal spray bottle.
  2. 2. Wipe the top of your peptide vial with an alcohol prep pad. Let dry.
  3. 3. Take a syringe out of the packaging and attach the needle.
  4. 4. Draw some air into the syringe and inject it into the peptide vial to release the air pressure.
  5. 5. Using the syringe, draw 1mL of saline out of the nasal spray bottle.
  6. 6. Slowly inject it into the peptide vial along the side of the glass. Do not inject directly onto the powder.
  7. 7. Gently roll the vial between your fingers until fully dissolved.
Step 02

Loading the Nasal Sprayer

  1. 1. Flip the vial upside-down and insert the syringe. Draw the reconstituted solution out of the vial.
  2. 2. Inject it back into the nasal spray bottle with the remaining 4mL of saline.
  3. 3. Replace the top and prime with 3-4 pumps until solution appears.
  4. 4. Use the included label to label your bottle with the peptide name, concentration, and date prepared.
Storage
Keep refrigerated at 36-46°F (2-8°C) when not in use. Reconstituted peptides stored in saline are generally stable for 2-4 weeks refrigerated.

These instructions are based on a 10mg peptide vial. Using 5mL of saline gives you 200mcg per spray and 50 total sprays. If your vial is a different size or you want a different concentration, use our free Intranasal Peptide Calculator to get the exact amounts for your setup.

Common Questions
How long do reconstituted peptides last in the fridge?
Reconstituted peptides stored in sterile saline are generally stable for 2-4 weeks when kept refrigerated at 36-46°F (2-8°C). Stability varies by peptide - Semax and Selank, for example, are considered relatively stable post-reconstitution. When in doubt, prepare fresh and keep refrigerated between uses.
How do I calculate the dose per spray for my peptide vial?
Each pump on the included nasal spray bottle delivers approximately 0.1mL. To find your dose per spray, divide the total peptide amount by the total volume in mL, then multiply by 0.1. For a 10mg vial in 5mL saline: (10,000mcg / 5mL) x 0.1mL = 200mcg per spray. Use our free calculator to run the math for any vial size or target dose.
Why use a luer lock syringe instead of an insulin syringe?
Insulin syringes have fixed needles, very fine gauges that slow draw speed, limited barrel volume, and short needles that make penetrating rubber-topped vials awkward. Luer lock syringes with removable needles give you more control - you can swap needle lengths, draw larger volumes cleanly, and transfer solution without losing material to dead space.